|
Status |
Public on Aug 31, 2016 |
Title |
ECT4 Post |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
ECT Post Tx Non-Responder
|
Organism |
Homo sapiens |
Characteristics |
disease state: MDD individual: ECT4 treatment: ECT time: post-treatment response: non-responder tissue: blood
|
Treatment protocol |
Patients were either treated with electroconvulsive therapy or ketamine infusions.
|
Growth protocol |
Not applicable.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted using manufacturer's intstruction.
|
Label |
Hy3
|
Label protocol |
400 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit (Exiqon, Denmark) following the procedure described by the manufacturer. This project was run with a dual colour setup and LOWES normalization so the reference RNA comprised a pool of RNA containing all samples in equal amounts.
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|
|
Channel 2 |
Source name |
Reference
|
Organism |
Homo sapiens |
Characteristics |
reference composition: a pool of RNA containing all samples in equal amounts
|
Treatment protocol |
Patients were either treated with electroconvulsive therapy or ketamine infusions.
|
Growth protocol |
Not applicable.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted using manufacturer's intstruction.
|
Label |
Hy5
|
Label protocol |
400 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit (Exiqon, Denmark) following the procedure described by the manufacturer. This project was run with a dual colour setup and LOWES normalization so the reference RNA comprised a pool of RNA containing all samples in equal amounts.
|
|
|
|
Hybridization protocol |
The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).
|
Scan protocol |
The miRCURY LNA™ microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon, Denmark).
|
Description |
Hy3: 1_Exiqon_E4POST.txt Hy5: 0_Exiqon_E4POST.txt Non-Responder
|
Data processing |
The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
|
|
Submission date |
May 05, 2016 |
Last update date |
Aug 31, 2016 |
Contact name |
Anand Gururajan |
Organization name |
University College Cork
|
Department |
Anatomy & Neuroscience
|
Street address |
Western Road
|
City |
Cork |
ZIP/Postal code |
NA |
Country |
Ireland |
|
|
Platform ID |
GPL21814 |
Series (1) |
GSE81152 |
MicroRNAs as diagnostic biomarkers for major depression |
|