NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2136954 Query DataSets for GSM2136954
Status Public on Feb 23, 2017
Title MOLM13.POL2_sgGFP
Sample type SRA
 
Source name MOLM13
Organism Homo sapiens
Characteristics cell line: MOLM13
treatment: Control
antibody: Santa Cruz sc-899X
Treatment protocol For Flag-tagged stable cell selection, 10 ug/ml blasticidin was used for stable cells selection. For iCas9 induced ENL CRISPR knockout, 1ug/ml of doxycycline was used for induction of 5days before collecting for ChIP.
Growth protocol Cells were maintained in RPMI with 10% FBS, 5% CO2, 37oC.
Extracted molecule genomic DNA
Extraction protocol ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20.
Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit according to the manufacturer's protocol. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250-450 bp (insert plus adaptor) were band isolated from an agarose gel. DNA fragments were sequenced using paired-end sequencing technology on Illumina HiSeq 2000 platform.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Data processing Basecalls performed by Illumina CASAVA 1.8.2
ChIP-seq reads were aligned to the hg19 genome using Bowtie2 v2.1.0
Genome_build: hg19
Supplementary_files_format_and_content: ChIP-seq wig files were generated using MACS-1.3.6+B102; Scores represent the ChIP-seq tag numbers
 
Submission date Apr 28, 2016
Last update date May 15, 2019
Contact name Hong Wen
Organization name Van Andel Research Institute
Department Center for Epigenetics
Street address 333 Bostwick Ave. N.E.
City Grand Rapids
State/province Michigan
ZIP/Postal code 49503
Country USA
 
Platform ID GPL18573
Series (1)
GSE80779 ENL Links Histone Acetylation to Oncogenic Gene Expression in AML
Relations
BioSample SAMN04913724
SRA SRX1735820

Supplementary file Size Download File type/resource
GSM2136954_MOLM13.POL2_sgGFP.wig.gz 32.3 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap