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Sample GSM2135641 Query DataSets for GSM2135641
Status Public on Apr 28, 2017
Title Male_Achl_RNAi_1
Sample type SRA
 
Source name heads
Organism Drosophila melanogaster
Characteristics tissue: heads
age: 3-5 day old
develpomental stage: adult
Growth protocol 3-5 day old adult flies were maintained on standard food at 25 degrees under a 12hr:12hr light:dark condition and samples were collected at ZT2.
Extracted molecule total RNA
Extraction protocol fly heads were manually dissected in PBS and homogenized in Ttizol (Life Technologies), total RNAs were collected using Phase lock gels (5Prime) and RNeasy mini kit (Qiagen)
libraries were prepared with 200ng total RNA per sample using TruSeq Stranded mRNA LT prep kit (Illumina)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Data processing RUM (Grant et al. 2011) was used to align raw reads to the reference Drosophila genome and transcriptome using the following parameters: “--strand-specific --variable-length-reads --bowtie-nu-limit 10 --nu-limit 10”.
Genome_build: dm3
 
Submission date Apr 28, 2016
Last update date May 15, 2019
Contact name Jiajia Li
E-mail(s) jli.umsl@gmail.com
Organization name University of Missouri-St. Louis
Department Biology
Lab Hughes Lab
Street address 435 Research Building, One University Boulevard
City St. Louis
State/province Missouri
ZIP/Postal code 63121
Country USA
 
Platform ID GPL16479
Series (1)
GSE80738 Achilles is a circadian clock controlled gene that regulates innate immune function in Drosophila
Relations
Reanalyzed by GSM3278932
BioSample SAMN04910099
SRA SRX1734344

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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