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Sample GSM2131184 Query DataSets for GSM2131184
Status Public on Jun 14, 2016
Title MK24h_2_s2_treat
Sample type SRA
Source name T47D breast cancer cells
Organism Homo sapiens
Characteristics cell type: T47D
treatment: AKT inhibitor MK2206
Treatment protocol The medium was changed to medium containing either MK2206 or DMSO for 24 hours.
Growth protocol Cells were maintained at 37oC and 5% CO2 in RPMI or DMEM + 10% FBS and Pen-Strep.
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy
Libraries were made from total RNA using the Illumina TruSeq Stranded mRNA Library Prep Kit
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Basecalls were performed with FastQC version 0.10.0.
RNA-seq reads were aligned to the hg19 genome assembly using Tophat
Genome_build: hg19
Submission date Apr 22, 2016
Last update date May 15, 2019
Contact name Koen M.A. Dreijerink
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL18573
Series (2)
GSE80591 Messenger RNA expression after MK2206/DMSO treatment of T47D cells
GSE80594 PI3K/AKT signaling regulates H3K4 methylation in breast cancer
BioSample SAMN04886744
SRA SRX1721559

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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