NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2126104 Query DataSets for GSM2126104
Status Public on Oct 11, 2016
Title MMTV_PyMT_369_Cell_Tum
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics tumor type: Breast cancer
model type: Genetically Engineered Model
cell type: Mouse tumor cells
mouse type: FVB
patient id: N/A
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
 
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer GEM, Tumor 369
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
 
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL11533
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10338001 10.67406
10338002 1.792167
10338003 8.819157
10338004 4.791222
10338005 1.051327
10338006 1.182808
10338007 1.31447
10338008 1.30261
10338009 4.252748
10338010 1.048928
10338011 1.575718
10338012 1.097359
10338013 0.9781718
10338014 0.9982883
10338015 0.9798059
10338016 2.488115
10338017 11.72559
10338018 1.933538
10338019 1.465638
10338020 3.871965

Total number of rows: 35556

Table truncated, full table size 622 Kbytes.




Supplementary file Size Download File type/resource
GSM2126104_MMTV_PyMT_369_Cell_Tum.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap