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Sample GSM2126103 Query DataSets for GSM2126103
Status Public on Oct 11, 2016
Title MMTV_PyMT_360_Macro
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics tumor type: Breast cancer
model type: Genetically Engineered Model
cell type: Mouse macrophages (M1+M2)
mouse type: FVB
patient id: N/A
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
 
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer GEM, Tumor 360
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
 
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL11533
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10338001 10.48165
10338002 1.649582
10338003 8.239049
10338004 4.508357
10338005 1.016187
10338006 1.07942
10338007 1.212558
10338008 1.232217
10338009 3.204472
10338010 1.001212
10338011 1.518769
10338012 1.059808
10338013 0.871533
10338014 0.911235
10338015 0.8559083
10338016 2.156398
10338017 11.83087
10338018 1.671478
10338019 1.38579
10338020 3.031745

Total number of rows: 35556

Table truncated, full table size 623 Kbytes.




Supplementary file Size Download File type/resource
GSM2126103_MMTV_PyMT_360_Macro.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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