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Sample GSM2126095 Query DataSets for GSM2126095
Status Public on Oct 11, 2016
Title BC_PyMT_18_Macro
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics tumor type: Breast cancer
model type: Genetically Engineered Model
cell type: Mouse macrophages (M1+M2)
mouse type: Nude
patient id: N/A
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
 
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer GEM, Tumor 18
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
 
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL11533
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10338001 10.71678
10338002 1.690191
10338003 8.696619
10338004 4.099868
10338005 1.051327
10338006 1.146034
10338007 1.260669
10338008 1.281782
10338009 3.549187
10338010 1.016649
10338011 1.545884
10338012 1.059363
10338013 0.9251509
10338014 0.9673415
10338015 0.9050775
10338016 2.17439
10338017 11.60571
10338018 1.737889
10338019 1.436424
10338020 3.191359

Total number of rows: 35556

Table truncated, full table size 622 Kbytes.




Supplementary file Size Download File type/resource
GSM2126095_BC_PyMT_18_Macro.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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