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Sample GSM2126085 Query DataSets for GSM2126085
Status Public on Oct 11, 2016
Title BC334_RO_2_Cell_Tum
Sample type RNA
 
Source name tumor
Organism Homo sapiens
Characteristics tumor type: Breast cancer
model type: Patient-Derived xenograft (PDX)
cell type: Human tumor cells
mouse type: Nude
patient id: RO
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
 
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer PDX n° 334, Tumor 2
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
 
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL11532
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 1.93948
7892502 0.9111972
7892503 2.307347
7892504 4.751294
7892505 1.739903
7892506 1.00379
7892507 2.387125
7892508 2.510079
7892509 8.041463
7892510 1.138483
7892511 0.7792672
7892512 2.096581
7892513 0.8500726
7892514 5.166367
7892515 3.358646
7892516 0.9552578
7892517 0.8635696
7892518 1.348033
7892519 1.308383
7892520 4.40503

Total number of rows: 33297

Table truncated, full table size 551 Kbytes.




Supplementary file Size Download File type/resource
GSM2126085_BC334_RO_2_Cell_Tum.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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