GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2126084 Query DataSets for GSM2126084
Status Public on Oct 11, 2016
Title BC334_RO_1_Cell_Tum
Sample type RNA
Source name tumor
Organism Homo sapiens
Characteristics tumor type: Breast cancer
model type: Patient-Derived xenograft (PDX)
cell type: Human tumor cells
mouse type: Nude
patient id: RO
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer PDX n° 334, Tumor 1
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL11532
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
VALUE RMA signal intensity

Data table
7892501 2.432993
7892502 1.086906
7892503 2.338079
7892504 4.581016
7892505 1.127637
7892506 1.951365
7892507 2.11712
7892508 0.9314471
7892509 6.673882
7892510 0.9846348
7892511 1.435639
7892512 1.229448
7892513 1.004688
7892514 4.692501
7892515 3.455833
7892516 1.051268
7892517 1.567805
7892518 1.30025
7892519 2.429882
7892520 4.654586

Total number of rows: 33297

Table truncated, full table size 550 Kbytes.

Supplementary file Size Download File type/resource
GSM2126084_BC334_RO_1_Cell_Tum.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap