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Sample GSM2126082 Query DataSets for GSM2126082
Status Public on Oct 11, 2016
Title BC256_LEV_37_Cell_Tum
Sample type RNA
Source name tumor
Organism Homo sapiens
Characteristics tumor type: Breast cancer
model type: Patient-Derived xenograft (PDX)
cell type: Human tumor cells
mouse type: Nude
patient id: LEV
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer PDX n° 256, Tumor 37
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL11532
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
VALUE RMA signal intensity

Data table
7892501 2.444285
7892502 1.211639
7892503 1.876167
7892504 4.692476
7892505 1.224571
7892506 0.7395789
7892507 3.904763
7892508 1.503876
7892509 7.883242
7892510 2.041469
7892511 0.9266756
7892512 1.676248
7892513 0.9204569
7892514 6.285413
7892515 3.610795
7892516 1.310782
7892517 1.179987
7892518 1.109539
7892519 1.720862
7892520 4.529944

Total number of rows: 33297

Table truncated, full table size 550 Kbytes.

Supplementary file Size Download File type/resource
GSM2126082_BC256_LEV_37_Cell_Tum.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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