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Sample GSM2126075 Query DataSets for GSM2126075
Status Public on Oct 11, 2016
Title BC52_EDW_p15_Frag
Sample type RNA
 
Source name tumor
Organism Homo sapiens
Characteristics tumor type: Breast cancer
model type: Patient-Derived xenograft (PDX)
cell type: All human tumor
mouse type: Nude
patient id: EDW
Treatment protocol Dissociation of tumors was performed using an procedure optimized for our tumors described in Petit et al. [ Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Nemati F, et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 2013].
Growth protocol Tumor and tumor-associated stromal cells were obtained after tissue dissociation of 1500mm3 tumors
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the sorted fractions using the RNAeasy Plus Micro kit (Qiagen, Inc.)
Label biotin
Label protocol RNA was amplified by WT expression kit (Ambion) and Biotinylated by WT terminal labeling kit (Affymetrix)
 
Hybridization protocol Following fragmentation, 5.5 ug of cDNA were hybridized for 17 hr at 48C on Human Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneTitan.
Scan protocol GeneChips were scanned in the Affymetrix GeneTitan.
Description Breast cancer PDX n° 52, in vivo passage 15
Data processing Data were normalized using Expression Console from Affymetrix (RMA-GENE analysis)
 
Submission date Apr 18, 2016
Last update date Oct 11, 2016
Contact name Pierre de la Grange
E-mail(s) pierre.delagrange@genosplice.com
Organization name GenoSplice technology
Department Paris Biotech Santé
Street address 29, rue du Faubourg Saint-Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL11532
Series (1)
GSE80410 Characterization of tumor-associated-macrophage of breast cancer patient-derived xenografts

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 2.434153
7892502 0.9039037
7892503 1.098166
7892504 4.426622
7892505 1.107989
7892506 1.027113
7892507 2.182335
7892508 1.232331
7892509 8.049578
7892510 1.261888
7892511 1.297328
7892512 1.056992
7892513 1.072996
7892514 6.191011
7892515 4.132429
7892516 1.738039
7892517 0.9033498
7892518 0.8647594
7892519 1.285485
7892520 4.428073

Total number of rows: 33297

Table truncated, full table size 551 Kbytes.




Supplementary file Size Download File type/resource
GSM2126075_BC52_EDW_p15_Frag.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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