|Public on Jun 16, 2016
|Lung carcinoma cells
|cell line: H157
cell type: Lung carcinoma cells
genotype/variation: expressing YAP-TEAD305
|The H157 lung carcinoma cells stably expressing different construct were collected after reaching mid-log growth phase, then subject to RNA purification
|The H157 lung carcinoma cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 1% penicillin/streptomycin.
|Total RNAs were purified from cells using Trizol method and subsequently cleaned using RNAeasy Kit.
Total RNA not exceeding 3 μg was further used to purify polyadenylated RNA using Illumina TruSeq Total RNA Sample Prep kits. We used the Ribo-Zero Human to remove cytoplasmic rRNA. The mRNA purified was further analyzed using Bioanalyzer (Agilent Technologies) prior to generation of cDNA library with bar coded ends. RNA-seq libraries were robotically prepared with Illumina TruSeq Total RNA Sample Prep kits according to the manufacturer’s protocol.
|Illumina NextSeq 500
|Sequenced reads were aligned to hg19 using Bowtie v2.2.5
The EBSeq tool was used to identify differentially expressed genes in pairwise comparisons of all conditions
supplementary_files_format_and_content: Gene expression level of all conditions was merged to genes-merge.counts.matrix using rsem-generate-data-matrix. Scores represent expected reads count at each condition derived from rsem-calculate-expression
|Apr 18, 2016
|Last update date
|May 15, 2019
|CAS-MPG Partner Institute for Computational Biology (PICB)
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|A splicing switch of TEAD4 regulates Hippo-YAP signaling pathway to inhibit tumor proliferation