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Sample GSM2113123 Query DataSets for GSM2113123
Status Public on Dec 19, 2017
Title 20_Ctrl_P10
Sample type RNA
 
Source name whole lung
Organism Rattus norvegicus
Characteristics age: P10 of life
sample origin: Ctrl diet during gestation
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Rneasy Qiagen kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
Label biotin
Label protocol After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
 
Hybridization protocol Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Data processing The data were preprocessed and analyzing using Partek Genomic Suite.
 
Submission date Apr 11, 2016
Last update date Dec 19, 2017
Contact name Céline Méhats
E-mail(s) celine.mehats@inserm.fr
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL19117
Series (1)
GSE80123 microRNA expression profile in whole lung of rat pups at P10 or P21 day of life after a intrauterine growth restriction due to a low protein diet during gestation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
14q0_st 0.482491
14qI-1_st 0.669526
14qI-1_x_st 0.849105
14qI-2_st 1.01328
14qI-3_x_st 0.512535
14qI-4_st 0.619051
14qI-4_x_st 0.510118
14qI-5_st 0.96093
14qI-6_st 0.247397
14qI-7_st 0.56149
14qI-8_st 0.759056
14qI-8_x_st 0.821103
14qI-9_x_st 0.596632
14qII-10_st 0.598216
14qII-11_st 0.813735
14qII-12_st 0.528882
14qII-12_x_st 0.516559
14qII-13_st 0.56964
14qII-14_st 0.71536
14qII-14_x_st 1.10116

Total number of rows: 36353

Table truncated, full table size 867 Kbytes.




Supplementary file Size Download File type/resource
GSM2113123_20_miRNA-4_0_.CEL.gz 714.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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