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Sample GSM2113111 Query DataSets for GSM2113111
Status Public on Dec 19, 2017
Title 8_Ctrl_P21
Sample type RNA
 
Source name whole lung
Organism Rattus norvegicus
Characteristics age: P21 of life
sample origin: Ctrl diet during gestation
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Rneasy Qiagen kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
Label biotin
Label protocol After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
 
Hybridization protocol Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Data processing The data were preprocessed and analyzing using Partek Genomic Suite.
 
Submission date Apr 11, 2016
Last update date Dec 19, 2017
Contact name Céline Méhats
E-mail(s) celine.mehats@inserm.fr
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL19117
Series (1)
GSE80123 microRNA expression profile in whole lung of rat pups at P10 or P21 day of life after a intrauterine growth restriction due to a low protein diet during gestation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
14q0_st 0.729824
14qI-1_st 0.900824
14qI-1_x_st 0.963201
14qI-2_st 0.68532
14qI-3_x_st 0.49566
14qI-4_st 0.786189
14qI-4_x_st 0.561118
14qI-5_st 0.50385
14qI-6_st 0.835209
14qI-7_st 1.43714
14qI-8_st 0.669137
14qI-8_x_st 0.855279
14qI-9_x_st 0.662054
14qII-10_st 0.78925
14qII-11_st 0.695216
14qII-12_st 1.22594
14qII-12_x_st 0.649516
14qII-13_st 0.806894
14qII-14_st 0.41375
14qII-14_x_st 1.18979

Total number of rows: 36353

Table truncated, full table size 867 Kbytes.




Supplementary file Size Download File type/resource
GSM2113111_8_miRNA-4_0_.CEL.gz 709.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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