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Sample GSM2113110 Query DataSets for GSM2113110
Status Public on Dec 19, 2017
Title 7_Ctrl_P21
Sample type RNA
 
Source name whole lung
Organism Rattus norvegicus
Characteristics age: P21 of life
sample origin: Ctrl diet during gestation
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Rneasy Qiagen kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
Label biotin
Label protocol After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
 
Hybridization protocol Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Data processing The data were preprocessed and analyzing using Partek Genomic Suite.
 
Submission date Apr 11, 2016
Last update date Dec 19, 2017
Contact name Céline Méhats
E-mail(s) celine.mehats@inserm.fr
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL19117
Series (1)
GSE80123 microRNA expression profile in whole lung of rat pups at P10 or P21 day of life after a intrauterine growth restriction due to a low protein diet during gestation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
14q0_st 0.71669
14qI-1_st 1.15205
14qI-1_x_st 1.15205
14qI-2_st 0.66495
14qI-3_x_st 0.472333
14qI-4_st 0.616763
14qI-4_x_st 0.787308
14qI-5_st 0.586739
14qI-6_st 0.553884
14qI-7_st 0.806175
14qI-8_st 0.773121
14qI-8_x_st 0.913346
14qI-9_x_st 0.908509
14qII-10_st 0.319445
14qII-11_st 1.06618
14qII-12_st 0.668202
14qII-12_x_st 0.937499
14qII-13_st 0.713948
14qII-14_st 0.664114
14qII-14_x_st 0.930565

Total number of rows: 36353

Table truncated, full table size 866 Kbytes.




Supplementary file Size Download File type/resource
GSM2113110_7_miRNA-4_0_.CEL.gz 713.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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