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Sample GSM2107724 Query DataSets for GSM2107724
Status Public on Sep 26, 2016
Title lnc-ASTN1-LNA1-rep1
Sample type SRA
Source name REH cell line
Organism Homo sapiens
Characteristics cell line: REH
treatment: lnc-ASTN1-1 LNA GapmeR1
time: 72h
Treatment protocol Cells were seeded at 1*10^6 cells/ml and treated with either medium or 10 ┬ÁM LNA GapmeRs. Cells were harvested 72h after treatment.
Growth protocol Cells were grown in RPMI supplemented with 20% fetal bovine serum, 1% glutamin and 1% penicilin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was harvested with the miRNeasy mini kit (Qiagen) with Dnase treatment on-column.
totalRNA, ribodepletion, stranded RNA-seq
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Aligned with STAR2.4.2a to hg38, using Ensembl38v81 as guide
Genome_build: hg38
Supplementary_files_format_and_content: STAR count output
Submission date Apr 04, 2016
Last update date May 15, 2019
Contact name Farzaneh Ghazavi
Organization name Gent university hospital
Department Center of medical genetics
Street address De Pintelaan 185
City Ghent
ZIP/Postal code 9000
Country Belgium
Platform ID GPL18573
Series (2)
GSE79872 RNA-sequencing of B-ALL cell lines, REH treated with LNA GapmeRs against four lncRNAs
GSE79873 Unique long non-coding RNA expression signature in ETV6/RUNX1-driven B-cell precursor acute lymphoblastic leukemia
BioSample SAMN04605057
SRA SRX1677215

Supplementary file Size Download File type/resource 321.7 Kb (ftp)(http) TAB
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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