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Sample GSM2107719 Query DataSets for GSM2107719
Status Public on Sep 26, 2016
Title lnc-TIMM21-LNA2-rep1
Sample type SRA
 
Source name REH cell line
Organism Homo sapiens
Characteristics cell line: REH
treatment: lnc-TIMM21-5 LNA GapmeR2
time: 72h
Treatment protocol Cells were seeded at 1*10^6 cells/ml and treated with either medium or 10 ┬ÁM LNA GapmeRs. Cells were harvested 72h after treatment.
Growth protocol Cells were grown in RPMI supplemented with 20% fetal bovine serum, 1% glutamin and 1% penicilin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was harvested with the miRNeasy mini kit (Qiagen) with Dnase treatment on-column.
totalRNA, ribodepletion, stranded RNA-seq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Aligned with STAR2.4.2a to hg38, using Ensembl38v81 as guide
Genome_build: hg38
Supplementary_files_format_and_content: STAR count output
 
Submission date Apr 04, 2016
Last update date May 15, 2019
Contact name Farzaneh Ghazavi
E-mail(s) farzaneh.ghazavi@ugent.be
Organization name Gent university hospital
Department Center of medical genetics
Street address De Pintelaan 185
City Ghent
ZIP/Postal code 9000
Country Belgium
 
Platform ID GPL18573
Series (2)
GSE79872 RNA-sequencing of B-ALL cell lines, REH treated with LNA GapmeRs against four lncRNAs
GSE79873 Unique long non-coding RNA expression signature in ETV6/RUNX1-driven B-cell precursor acute lymphoblastic leukemia
Relations
BioSample SAMN04605052
SRA SRX1677210

Supplementary file Size Download File type/resource
GSM2107719_sample_4_ReadsPerGene.out.tab.gz 326.2 Kb (ftp)(http) TAB
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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