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Sample GSM210750 Query DataSets for GSM210750
Status Public on Jul 15, 2008
Title Cap_Gata4_GR+Dkk1_rep3
Sample type RNA
 
Source name Gata4+Dkk1 injected animal cap_CHX+Dex_2.5 hours.
Organism Xenopus laevis
Characteristics Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
Treatment protocol 10ng of inducible mRNA construct was injected into one cell embryos at around 20 degrees off the animal pole.
Growth protocol Embryos were injected with 10ng of each mRNA at the one cell stage and allowed to reach stage 9, whereupon animal caps were excised and cultured in .75 NAM for 2.5 hours in the presence of cycloheximide and dexamethasone.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Xenopus leavis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanned using manufacturer's protocols.
Description Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
Data processing Data were normalised and analysed using Genespring 7.0 using default settings.
 
Submission date Jul 16, 2007
Last update date Aug 14, 2011
Contact name Kathryn Maddison
Organization name Cardiff University
Department Genetics
Street address Museum Avenue
City Cardiff
ZIP/Postal code CF14 2HB
Country United Kingdom
 
Platform ID GPL1318
Series (1)
GSE8496 Examining the immediate early targets of Gata4 and Gata5.

Data table header descriptions
ID_REF
VALUE MAS-5.0
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 92.7 P 0.000754
AFFX-BioB-5_at 175.6 P 0.000052
AFFX-BioB-M_at 127.6 P 0.000044
AFFX-BioC-3_at 332.8 P 0.000044
AFFX-BioC-5_at 348.2 P 0.000044
AFFX-BioDn-3_at 684.2 P 0.000044
AFFX-BioDn-5_at 1161.8 P 0.000044
AFFX-CreX-3_at 4506.4 P 0.000044
AFFX-CreX-5_at 6625.8 P 0.000044
AFFX-DapX-3_at 39.5 P 0.000081
AFFX-DapX-5_at 88.6 P 0.000857
AFFX-DapX-M_at 143.6 P 0.000081
AFFX-LysX-3_at 7.3 A 0.095667
AFFX-LysX-5_at 23.9 A 0.095667
AFFX-LysX-M_at 39.8 P 0.000127
AFFX-PheX-3_at 13.2 P 0.012547
AFFX-PheX-5_at 14.1 A 0.0727
AFFX-PheX-M_at 23.9 P 0.020022
AFFX-r2-Bs-dap-3_at 16.1 M 0.042962
AFFX-r2-Bs-dap-5_at 31.1 P 0.010317

Total number of rows: 15611

Table truncated, full table size 497 Kbytes.




Supplementary file Size Download File type/resource
GSM210750.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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