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Sample GSM2097498 Query DataSets for GSM2097498
Status Public on Mar 01, 2019
Title Control TAC 3
Sample type RNA
Source name Control Heart tissue 21 Days after surgery TAC
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: wild type
treatment: trans aortic banding
time: 21
Treatment protocol The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
Growth protocol Mice were sacrificed 21 days after surgery (Sham and TAC).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Mini Kit using DNAse treatment
Label Cy3
Label protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
Hybridization protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
Scan protocol Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version (Agilent Technologies)
Data processing Data was normaliced with RMA in GeneSpring software.
Submission date Mar 23, 2016
Last update date Mar 01, 2019
Contact name Jesus Maria Gómez-Salnero
Phone (+34) 91 4531200
Organization name CNIC
Street address Melchor Fernández Almagro, 3
City Madrid
ZIP/Postal code 28007
Country Spain
Platform ID GPL11202
Series (1)
GSE79536 The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
GE_BrightCorner -0.6646533
DarkCorner 0.40331936
A_55_P1989846 0.11658096
A_55_P1991598 -0.042905807
A_55_P2022211 1.3540626
A_55_P1980764 0.22192049
A_55_P1964375 -0.20926476
A_51_P128876 0.23219204
A_55_P2121042 -0.7718086
A_52_P219230 0.13808155
A_51_P207591 1.9076998
A_55_P2131920 0.088748455
A_55_P2404223 -0.10577726
A_55_P2101944 -0.4407978
A_52_P358860 0.12813425
A_51_P119031 0.005427837
A_51_P309854 -0.6522093
A_51_P343900 -0.41345596
A_51_P234359 -0.004768372
A_51_P487813 1.1337886

Total number of rows: 39485

Table truncated, full table size 958 Kbytes.

Supplementary file Size Download File type/resource
GSM2097498_WT_TAC_97B.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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