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Sample GSM2097496 Query DataSets for GSM2097496
Status Public on Mar 01, 2019
Title Control TAC 1
Sample type RNA
 
Source name Control Heart tissue 21 Days after surgery TAC
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: wild type
treatment: trans aortic banding
time: 21
Treatment protocol The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
Growth protocol Mice were sacrificed 21 days after surgery (Sham and TAC).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Mini Kit using DNAse treatment
Label Cy3
Label protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
 
Hybridization protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
Scan protocol Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version 9.5.3.1 (Agilent Technologies)
Data processing Data was normaliced with RMA in GeneSpring software.
 
Submission date Mar 23, 2016
Last update date Mar 01, 2019
Contact name Jesus Maria Gómez-Salnero
E-mail(s) jmgomez@cnic.es
Phone (+34) 91 4531200
Organization name CNIC
Street address Melchor Fernández Almagro, 3
City Madrid
ZIP/Postal code 28007
Country Spain
 
Platform ID GPL11202
Series (1)
GSE79536 The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.45441294
DarkCorner -0.69909096
A_55_P1989846 0.026082039
A_55_P1991598 0.37819624
A_55_P2022211 0.9985776
A_55_P1980764 0.19914532
A_55_P1964375 -0.23214054
A_51_P128876 0.13318539
A_55_P2121042 -0.98848915
A_52_P219230 -0.50184345
A_51_P207591 2.1614506
A_55_P2131920 0.18636465
A_55_P2404223 0.013947964
A_55_P2101944 0.7022295
A_52_P358860 -0.1603837
A_51_P119031 -0.024868488
A_51_P309854 -0.39978695
A_51_P343900 -0.16657925
A_51_P234359 0.30398417
A_51_P487813 0.99509573

Total number of rows: 39485

Table truncated, full table size 964 Kbytes.




Supplementary file Size Download File type/resource
GSM2097496_WT_TAC_66C.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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