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Sample GSM2097489 Query DataSets for GSM2097489
Status Public on Mar 01, 2019
Title Transgenic Sham 3
Sample type RNA
 
Source name Transgenic Heart tissue 21 Days after surgery NO TAC
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: CnAb1 transgenic overexpressing
treatment: sham
time: 0
Treatment protocol The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
Growth protocol Mice were sacrificed 21 days after surgery (Sham and TAC).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Mini Kit using DNAse treatment
Label Cy3
Label protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
 
Hybridization protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
Scan protocol Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version 9.5.3.1 (Agilent Technologies)
Data processing Data was normaliced with RMA in GeneSpring software.
 
Submission date Mar 23, 2016
Last update date Mar 01, 2019
Contact name Jesus Maria Gómez-Salnero
E-mail(s) jmgomez@cnic.es
Phone (+34) 91 4531200
Organization name CNIC
Street address Melchor Fernández Almagro, 3
City Madrid
ZIP/Postal code 28007
Country Spain
 
Platform ID GPL11202
Series (1)
GSE79536 The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.12732649
DarkCorner -0.1473484
A_55_P1989846 -0.26676273
A_55_P1991598 -1.7143812
A_55_P2022211 -0.2704568
A_55_P1980764 -0.14685154
A_55_P1964375 0.22233582
A_51_P128876 -0.025042534
A_55_P2121042 -1.2920742
A_52_P219230 -0.37877178
A_51_P207591 -0.27410722
A_55_P2131920 -0.35714102
A_55_P2404223 0.29176092
A_55_P2101944 0.95718384
A_52_P358860 0.7350888
A_51_P119031 -0.028247356
A_51_P309854 -0.5214863
A_51_P343900 0.55537224
A_51_P234359 0.001264095
A_51_P487813 -0.37633848

Total number of rows: 39485

Table truncated, full table size 965 Kbytes.




Supplementary file Size Download File type/resource
GSM2097489_B1_SHAM_51D.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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