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Sample GSM2097487 Query DataSets for GSM2097487
Status Public on Mar 01, 2019
Title Transgenic Sham 1
Sample type RNA
Source name Transgenic Heart tissue 21 Days after surgery NO TAC
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: CnAb1 transgenic overexpressing
treatment: sham
time: 0
Treatment protocol The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
Growth protocol Mice were sacrificed 21 days after surgery (Sham and TAC).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Mini Kit using DNAse treatment
Label Cy3
Label protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
Hybridization protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
Scan protocol Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version (Agilent Technologies)
Data processing Data was normaliced with RMA in GeneSpring software.
Submission date Mar 23, 2016
Last update date Mar 01, 2019
Contact name Jesus Maria Gómez-Salnero
Phone (+34) 91 4531200
Organization name CNIC
Street address Melchor Fernández Almagro, 3
City Madrid
ZIP/Postal code 28007
Country Spain
Platform ID GPL11202
Series (1)
GSE79536 The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
GE_BrightCorner 0.09473848
DarkCorner -0.009006977
A_55_P1989846 -0.09709358
A_55_P1991598 0.12447977
A_55_P2022211 -0.08171606
A_55_P1980764 0.013367653
A_55_P1964375 -8.79E-04
A_51_P128876 0.007236481
A_55_P2121042 0.4566307
A_52_P219230 0.34519434
A_51_P207591 0.31468558
A_55_P2131920 0.00719595
A_55_P2404223 0.15744734
A_55_P2101944 -0.24067497
A_52_P358860 -0.014322758
A_51_P119031 -0.005427837
A_51_P309854 0.20632648
A_51_P343900 -0.03606224
A_51_P234359 0.22285414
A_51_P487813 0.27343607

Total number of rows: 39485

Table truncated, full table size 967 Kbytes.

Supplementary file Size Download File type/resource
GSM2097487_B1_SHAM_24B.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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