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Sample GSM2094364 Query DataSets for GSM2094364
Status Public on Nov 23, 2016
Title siADNP rep1
Sample type SRA
Source name siADNP rep1
Organism Homo sapiens
Characteristics tissue: colon cancer
cell line: HCT116
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the Trizol standard protocol and treated with heat-labile dsDNAse (Thermo scientific)
libraries were produced from 150ng total RNA with Nugen complete RNAseq library system according to the manufacturers protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description siADPN1
Data processing Basecalling was done by RTA1.18.64
Truseq adapter and polyA were removed
reads were aligned to the human genome by STAR RNAseq read mapper
reads overlapping with annotated genes were counted with HTseq count
variance stabilisation and differential gene expression were done by DESeq2
Genome_build: hg19
Supplementary_files_format_and_content: raw count table from HTseq count and vst tranformed expression values from DEseq2
Submission date Mar 18, 2016
Last update date May 15, 2019
Contact name Stefan Krebs
Phone 0049-89-2180 76715
Organization name Ludwig-Maximilian University
Department Gene Center
Lab Lafuga Genomics
Street address Feodor-Lynen-Str. 25
City Munich
ZIP/Postal code 81377
Country Germany
Platform ID GPL18460
Series (1)
GSE79395 Knockdown of ADNP in HCT116 colon cancer cells
BioSample SAMN04568484
SRA SRX1643429

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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