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Sample GSM2061658 Query DataSets for GSM2061658
Status Public on Feb 16, 2016
Title ERalpha ChIP-seq LTED cells
Sample type SRA
Source name LTED breast cancer cells
Organism Homo sapiens
Characteristics cell line: LTED
chip antibody: ERα antibody (HC-20) (Santa Cruz, catalog# sc-543)
passage: <10
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
NEB ultra kit
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Data processing Basecalls performed using CASAVA version 1.4
Alignment was performed with Bowtie v1.1.2
Peak calling was perfromed using MACS 2.0
Genome_build: Hg19
Supplementary_files_format_and_content: bed file reports peaks
Submission date Feb 16, 2016
Last update date May 15, 2019
Contact name Luca Magnani
Organization name Imperial College London
Department Surgery and Cancer
Lab Magnani's Lab
Street address DuCane road
City London
ZIP/Postal code W120NN
Country United Kingdom
Platform ID GPL16791
Series (1)
GSE60517 Drug specific epigenetic reprogramming leads to increased cellular invasion in ERα positive breast cancer via de novo cholesterol biosynthesis.
SRA SRX1585244
BioSample SAMN04504414

Supplementary file Size Download File type/resource
GSM2061658_ERa_LTED_Darbre_peaks.bed.gz 285.2 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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