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Sample GSM2061433 Query DataSets for GSM2061433
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_75]
Sample type RNA
Source name aPD1+aCTLA4-pre sequential therapy sample (R1)
Organism Homo sapiens
Characteristics gender: F
individual: R
agent: Seq
time point: pre sequential therapy sample
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 7.4060187
JUC01000007.hg.1 5.756807
JUC01000009.hg.1 4.685366
JUC01000010.hg.1 9.848482
JUC01000023.hg.1 6.713383
JUC01000024.hg.1 4.9802556
JUC01000026.hg.1 7.21003
JUC01000028.hg.1 5.105827
JUC01000032.hg.1 4.820833
JUC01000033.hg.1 6.0698895
JUC01000039.hg.1 6.4251666
JUC01000040.hg.1 6.57669
JUC01000054.hg.1 6.4705033
JUC01000056.hg.1 7.168689
JUC01000074.hg.1 6.750016
JUC01000090.hg.1 7.0639
JUC01000108.hg.1 7.3708234
JUC01000109.hg.1 7.1232305
JUC01000151.hg.1 7.054051
JUC01000158.hg.1 6.3212967

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2061433_Sample_75.CEL.gz 25.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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