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Sample GSM2061428 Query DataSets for GSM2061428
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_70]
Sample type RNA
Source name aPD1+aCTLA4-post (O2)
Organism Homo sapiens
Characteristics gender: F
individual: O
agent: Combo
time point: post
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Inadequate RNA sample for aPD1+aCTLA4-pre (O1)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.5341144
JUC01000007.hg.1 5.6553974
JUC01000009.hg.1 4.6589894
JUC01000010.hg.1 9.930663
JUC01000023.hg.1 6.492966
JUC01000024.hg.1 5.0801272
JUC01000026.hg.1 7.078543
JUC01000028.hg.1 5.0852933
JUC01000032.hg.1 4.711396
JUC01000033.hg.1 6.074338
JUC01000039.hg.1 6.480982
JUC01000040.hg.1 6.2887735
JUC01000054.hg.1 6.5374804
JUC01000056.hg.1 7.2380996
JUC01000074.hg.1 6.895731
JUC01000090.hg.1 7.199142
JUC01000108.hg.1 7.5095205
JUC01000109.hg.1 7.2415285
JUC01000151.hg.1 6.890822
JUC01000158.hg.1 6.4786277

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2061428_Sample_70.CEL.gz 24.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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