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Sample GSM2061423 Query DataSets for GSM2061423
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_64]
Sample type RNA
 
Source name aPD1+aCTLA4-post sequential therapy sample (L2)
Organism Homo sapiens
Characteristics gender: M
individual: L
agent: Seq
time point: post sequential therapy sample
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
 
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
 
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
E-mail(s) rakesh.verma@yale.edu
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
JUC01000001.hg.1 6.4545226
JUC01000007.hg.1 5.564343
JUC01000009.hg.1 4.7044992
JUC01000010.hg.1 9.359331
JUC01000023.hg.1 6.319409
JUC01000024.hg.1 5.025554
JUC01000026.hg.1 7.2324557
JUC01000028.hg.1 5.095166
JUC01000032.hg.1 4.726355
JUC01000033.hg.1 6.1484456
JUC01000039.hg.1 6.3733597
JUC01000040.hg.1 6.409814
JUC01000054.hg.1 6.5047626
JUC01000056.hg.1 7.1996465
JUC01000074.hg.1 6.8597383
JUC01000090.hg.1 7.089485
JUC01000108.hg.1 7.2044683
JUC01000109.hg.1 7.0960655
JUC01000151.hg.1 6.7883334
JUC01000158.hg.1 6.376587

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.




Supplementary file Size Download File type/resource
GSM2061423_Sample_64.CEL.gz 24.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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