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Sample GSM2061422 Query DataSets for GSM2061422
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_63]
Sample type RNA
Source name aPD1+aCTLA4-pre sequential therapy sample (L1)
Organism Homo sapiens
Characteristics gender: M
individual: L
agent: Seq
time point: pre sequential therapy sample
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.491298
JUC01000007.hg.1 5.660355
JUC01000009.hg.1 4.779729
JUC01000010.hg.1 9.130935
JUC01000023.hg.1 6.336271
JUC01000024.hg.1 5.1230392
JUC01000026.hg.1 7.1566095
JUC01000028.hg.1 5.2294126
JUC01000032.hg.1 4.8422914
JUC01000033.hg.1 6.274114
JUC01000039.hg.1 6.511828
JUC01000040.hg.1 6.2484736
JUC01000054.hg.1 6.517304
JUC01000056.hg.1 7.359179
JUC01000074.hg.1 7.043147
JUC01000090.hg.1 7.227971
JUC01000108.hg.1 7.2075315
JUC01000109.hg.1 7.234056
JUC01000151.hg.1 6.9904447
JUC01000158.hg.1 6.529664

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2061422_Sample_63.CEL.gz 24.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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