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Sample GSM2061421 Query DataSets for GSM2061421
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_62]
Sample type RNA
Source name aCTLA4-post (K2)
Organism Homo sapiens
Characteristics gender: M
individual: K
agent: aCTLA4
time point: post
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.553583
JUC01000007.hg.1 5.663625
JUC01000009.hg.1 4.7526603
JUC01000010.hg.1 9.962514
JUC01000023.hg.1 6.761038
JUC01000024.hg.1 4.9940658
JUC01000026.hg.1 7.38877
JUC01000028.hg.1 5.098998
JUC01000032.hg.1 4.8616695
JUC01000033.hg.1 6.1178327
JUC01000039.hg.1 6.5957174
JUC01000040.hg.1 6.393718
JUC01000054.hg.1 6.4334126
JUC01000056.hg.1 7.169711
JUC01000074.hg.1 6.869604
JUC01000090.hg.1 7.1207905
JUC01000108.hg.1 7.2794843
JUC01000109.hg.1 7.118029
JUC01000151.hg.1 6.829538
JUC01000158.hg.1 6.2581577

Total number of rows: 67539

Table truncated, full table size 1757 Kbytes.

Supplementary file Size Download File type/resource
GSM2061421_Sample_62.CEL.gz 25.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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