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Sample GSM2061418 Query DataSets for GSM2061418
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_59]
Sample type RNA
Source name aPD1-pre (J1)
Organism Homo sapiens
Characteristics gender: M
individual: J
agent: aPD1
time point: pre
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.685202
JUC01000007.hg.1 5.7042294
JUC01000009.hg.1 4.9883895
JUC01000010.hg.1 9.720198
JUC01000023.hg.1 6.494585
JUC01000024.hg.1 5.0011654
JUC01000026.hg.1 6.945127
JUC01000028.hg.1 5.208672
JUC01000032.hg.1 4.93657
JUC01000033.hg.1 6.2748814
JUC01000039.hg.1 6.4328637
JUC01000040.hg.1 6.318989
JUC01000054.hg.1 6.5645185
JUC01000056.hg.1 7.3475323
JUC01000074.hg.1 6.9768286
JUC01000090.hg.1 7.2268414
JUC01000108.hg.1 7.2334986
JUC01000109.hg.1 7.2882953
JUC01000151.hg.1 6.9713836
JUC01000158.hg.1 6.5617137

Total number of rows: 67539

Table truncated, full table size 1757 Kbytes.

Supplementary file Size Download File type/resource
GSM2061418_Sample_59.CEL.gz 24.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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