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Sample GSM2061414 Query DataSets for GSM2061414
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_55]
Sample type RNA
Source name aCTLA4-pre (H1)
Organism Homo sapiens
Characteristics gender: F
individual: H
agent: aCTLA4
time point: pre
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.9479156
JUC01000007.hg.1 5.6641397
JUC01000009.hg.1 4.683224
JUC01000010.hg.1 9.505749
JUC01000023.hg.1 5.641869
JUC01000024.hg.1 5.135602
JUC01000026.hg.1 6.6641793
JUC01000028.hg.1 5.1491714
JUC01000032.hg.1 4.7201533
JUC01000033.hg.1 6.210127
JUC01000039.hg.1 6.5350614
JUC01000040.hg.1 6.323443
JUC01000054.hg.1 6.485501
JUC01000056.hg.1 7.398571
JUC01000074.hg.1 7.038485
JUC01000090.hg.1 7.2485843
JUC01000108.hg.1 7.1918116
JUC01000109.hg.1 7.2603602
JUC01000151.hg.1 7.0323343
JUC01000158.hg.1 6.4375377

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2061414_Sample_55.CEL.gz 24.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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