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Sample GSM2061409 Query DataSets for GSM2061409
Status Public on Feb 16, 2016
Title Human Peripheral Blood monocytes [Sample_50]
Sample type RNA
Source name aCTLA4-post (E2)
Organism Homo sapiens
Characteristics gender: M
individual: E
agent: aCTLA4
time point: post
cell type: Peripheral Blood monocytes
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood monocytes using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix) at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 15, 2016
Last update date Feb 16, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77924 Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE RMA signal

Data table
JUC01000001.hg.1 6.299549
JUC01000007.hg.1 5.7179847
JUC01000009.hg.1 4.6860075
JUC01000010.hg.1 9.900112
JUC01000023.hg.1 6.3595457
JUC01000024.hg.1 5.039829
JUC01000026.hg.1 7.220311
JUC01000028.hg.1 5.089658
JUC01000032.hg.1 4.7383375
JUC01000033.hg.1 6.073613
JUC01000039.hg.1 6.3938465
JUC01000040.hg.1 6.3115473
JUC01000054.hg.1 6.45883
JUC01000056.hg.1 7.311649
JUC01000074.hg.1 6.921449
JUC01000090.hg.1 7.197664
JUC01000108.hg.1 7.60254
JUC01000109.hg.1 7.260074
JUC01000151.hg.1 6.9009447
JUC01000158.hg.1 6.4655414

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2061409_Sample_50.CEL.gz 24.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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