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Sample GSM2056989 Query DataSets for GSM2056989
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_38]
Sample type RNA
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: F
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: post
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1+aCTLA4-post
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE normalized data

Data table
JUC01000001.hg.1 6.521265
JUC01000007.hg.1 5.5154424
JUC01000009.hg.1 4.639927
JUC01000010.hg.1 8.284355
JUC01000023.hg.1 6.254612
JUC01000024.hg.1 5.060287
JUC01000026.hg.1 7.0717745
JUC01000028.hg.1 5.3240123
JUC01000032.hg.1 4.6983457
JUC01000033.hg.1 6.197922
JUC01000039.hg.1 6.336365
JUC01000040.hg.1 7.0590267
JUC01000054.hg.1 6.382055
JUC01000056.hg.1 7.256012
JUC01000074.hg.1 6.870339
JUC01000090.hg.1 7.087087
JUC01000108.hg.1 7.143016
JUC01000109.hg.1 7.100228
JUC01000151.hg.1 6.8384147
JUC01000158.hg.1 6.1729755

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2056989_Sample_38.CEL.gz 24.2 Mb (ftp)(http) CEL
GSM2056989_Sample_38.chp.gz 457.4 Kb (ftp)(http) CHP
Processed data included within Sample table

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