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Sample GSM2056984 Query DataSets for GSM2056984
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_33]
Sample type RNA
 
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: M
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: pre
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1+aCTLA4-pre
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
 
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
E-mail(s) rakesh.verma@yale.edu
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
ID_REF
VALUE normalized data

Data table
ID_REF VALUE
JUC01000001.hg.1 6.594609
JUC01000007.hg.1 5.6067643
JUC01000009.hg.1 4.679256
JUC01000010.hg.1 8.703908
JUC01000023.hg.1 6.4630337
JUC01000024.hg.1 5.0981297
JUC01000026.hg.1 7.309975
JUC01000028.hg.1 5.3666635
JUC01000032.hg.1 4.75544
JUC01000033.hg.1 6.2565756
JUC01000039.hg.1 6.606217
JUC01000040.hg.1 7.144882
JUC01000054.hg.1 6.615221
JUC01000056.hg.1 7.4268837
JUC01000074.hg.1 7.012873
JUC01000090.hg.1 7.2469277
JUC01000108.hg.1 7.248744
JUC01000109.hg.1 7.287319
JUC01000151.hg.1 6.89438
JUC01000158.hg.1 6.4543314

Total number of rows: 67539

Table truncated, full table size 1757 Kbytes.




Supplementary file Size Download File type/resource
GSM2056984_Sample_33.CEL.gz 23.8 Mb (ftp)(http) CEL
GSM2056984_Sample_33.chp.gz 457.0 Kb (ftp)(http) CHP
Processed data included within Sample table

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