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Sample GSM2056975 Query DataSets for GSM2056975
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_24]
Sample type RNA
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: M
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: post
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1+aCTLA4-post
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE normalized data

Data table
JUC01000001.hg.1 6.3482876
JUC01000007.hg.1 5.464298
JUC01000009.hg.1 4.6555686
JUC01000010.hg.1 8.747502
JUC01000023.hg.1 6.3947773
JUC01000024.hg.1 5.0263214
JUC01000026.hg.1 7.116235
JUC01000028.hg.1 5.204469
JUC01000032.hg.1 4.7277513
JUC01000033.hg.1 6.125347
JUC01000039.hg.1 6.182865
JUC01000040.hg.1 7.344319
JUC01000054.hg.1 6.3658695
JUC01000056.hg.1 7.1899095
JUC01000074.hg.1 6.7390695
JUC01000090.hg.1 6.995382
JUC01000108.hg.1 6.9475584
JUC01000109.hg.1 7.021421
JUC01000151.hg.1 6.8660755
JUC01000158.hg.1 6.2721577

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2056975_Sample_24.CEL.gz 24.8 Mb (ftp)(http) CEL
GSM2056975_Sample_24.chp.gz 457.1 Kb (ftp)(http) CHP
Processed data included within Sample table

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