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Sample GSM2056969 Query DataSets for GSM2056969
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_18]
Sample type RNA
 
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: F
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: post
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1+aCTLA4-post
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
 
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
E-mail(s) rakesh.verma@yale.edu
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
ID_REF
VALUE normalized data

Data table
ID_REF VALUE
JUC01000001.hg.1 6.6029906
JUC01000007.hg.1 5.389722
JUC01000009.hg.1 4.783495
JUC01000010.hg.1 8.483909
JUC01000023.hg.1 6.5868006
JUC01000024.hg.1 4.937145
JUC01000026.hg.1 7.141667
JUC01000028.hg.1 5.18972
JUC01000032.hg.1 4.6413636
JUC01000033.hg.1 6.167031
JUC01000039.hg.1 6.4660106
JUC01000040.hg.1 7.7986374
JUC01000054.hg.1 6.3236594
JUC01000056.hg.1 7.072688
JUC01000074.hg.1 6.8619676
JUC01000090.hg.1 7.020721
JUC01000108.hg.1 7.3431826
JUC01000109.hg.1 7.0484056
JUC01000151.hg.1 6.795137
JUC01000158.hg.1 6.172513

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.




Supplementary file Size Download File type/resource
GSM2056969_Sample_18.CEL.gz 24.6 Mb (ftp)(http) CEL
GSM2056969_Sample_18.chp.gz 457.5 Kb (ftp)(http) CHP
Processed data included within Sample table

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