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Sample GSM2056963 Query DataSets for GSM2056963
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_12]
Sample type RNA
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: M
tissue: Peripheral Blood T cells
agent: aPD1+aCTLA4
time point: post
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1+aCTLA4-post
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE normalized data

Data table
JUC01000001.hg.1 6.7250395
JUC01000007.hg.1 5.565765
JUC01000009.hg.1 4.677255
JUC01000010.hg.1 8.697265
JUC01000023.hg.1 6.7029467
JUC01000024.hg.1 5.082016
JUC01000026.hg.1 7.3520727
JUC01000028.hg.1 5.2519836
JUC01000032.hg.1 4.8266582
JUC01000033.hg.1 6.187751
JUC01000039.hg.1 6.425373
JUC01000040.hg.1 6.845058
JUC01000054.hg.1 6.4349904
JUC01000056.hg.1 7.2080617
JUC01000074.hg.1 6.838132
JUC01000090.hg.1 7.0972085
JUC01000108.hg.1 7.1412983
JUC01000109.hg.1 7.0446267
JUC01000151.hg.1 6.8090925
JUC01000158.hg.1 6.2689147

Total number of rows: 67539

Table truncated, full table size 1757 Kbytes.

Supplementary file Size Download File type/resource
GSM2056963_Sample_12.CEL.gz 24.9 Mb (ftp)(http) CEL
GSM2056963_Sample_12.chp.gz 457.3 Kb (ftp)(http) CHP
Processed data included within Sample table

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