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Sample GSM2056953 Query DataSets for GSM2056953
Status Public on Feb 10, 2016
Title Human Peripheral Blood T cells [Sample_2]
Sample type RNA
Source name Human Peripheral Blood T cells
Organism Homo sapiens
Characteristics gender: M
tissue: Peripheral Blood T cells
agent: aPD1
time point: post
Growth protocol Not Applicable
Extracted molecule total RNA
Extraction protocol RNA was isolated from sorted peripheral blood T cells using RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip® Human Transcriptome Array 2.0 (Affymetrix) and scanned at the Yale Center for Genome Anlaysis (YCGA) Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description aPD1-post
Data processing Data on coding genes were analyzed using Genespring GX 12.5 platform. Data were imported via exon expression workflow employing RMA16 or PLIER16 (normalization) for analyzing the coding genes using the specific annotation support file for human transcriptome array 2.0 (as provided by Genespring GX).
Submission date Feb 09, 2016
Last update date Feb 10, 2016
Contact name Rakesh Verma
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
Platform ID GPL17585
Series (1)
GSE77714 Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Data table header descriptions
VALUE normalized data

Data table
JUC01000001.hg.1 6.81894
JUC01000007.hg.1 5.47137
JUC01000009.hg.1 4.629673
JUC01000010.hg.1 8.5651455
JUC01000023.hg.1 6.588435
JUC01000024.hg.1 4.9874325
JUC01000026.hg.1 7.0119123
JUC01000028.hg.1 5.191624
JUC01000032.hg.1 4.687064
JUC01000033.hg.1 6.162473
JUC01000039.hg.1 6.2969637
JUC01000040.hg.1 6.9603786
JUC01000054.hg.1 6.410702
JUC01000056.hg.1 7.052518
JUC01000074.hg.1 6.678914
JUC01000090.hg.1 6.972515
JUC01000108.hg.1 6.763467
JUC01000109.hg.1 6.9512115
JUC01000151.hg.1 6.761352
JUC01000158.hg.1 6.150186

Total number of rows: 67539

Table truncated, full table size 1756 Kbytes.

Supplementary file Size Download File type/resource
GSM2056953_Sample_2.CEL.gz 25.4 Mb (ftp)(http) CEL
GSM2056953_Sample_2.chp.gz 457.7 Kb (ftp)(http) CHP
Processed data included within Sample table

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