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Sample GSM2055157 Query DataSets for GSM2055157
Status Public on Feb 06, 2016
Title FFPE specimen of NFPA, biological rep3 [exon-level]
Sample type RNA
 
Source name obtained from archived tissue samples derived from patients with NFPA
Organism Homo sapiens
Characteristics gender: male
tissue: pituitary
age: 58 years old
Extracted molecule total RNA
Extraction protocol RNA was extracted by Trizol (Life Technology, 15596-018) following the manufacturer's instructions
Label biotin
Label protocol Total RNA were amplified, labeled and purified by using WT Terminal Labeling and Controls Kit (Cat#901524, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
 
Hybridization protocol Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 640 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions
Scan protocol Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 4.0 (Affymetrix, Santa Clara, CA, US) with default settings.
Description Gene expression data from FFPE specimen
Data processing Affymetrix GeneChip Command Console (version 4.0, Affymetrix) software was used to extract raw data. Next, Expression Console (version1.3.1, Affymetrix) software offered RMA normalization for both gene and exon level analysis. Then the gene expression analysis and alternative splice analysis proceeded by Genesrping software (version 13.0; Agilent Technologies) and Transcriptome Analysis Console (version2.0, Affymetrix) separately.
 
Submission date Feb 05, 2016
Last update date Feb 06, 2016
Contact name weikang xing
E-mail(s) xingweikang123@sina.com
Organization name The First Affiliated Hospital of Soochow University
Street address 188 shizi street
City suzhou
ZIP/Postal code 215000
Country China
 
Platform ID GPL17585
Series (1)
GSE77517 Genome-wide evaluation of change in lncRNA expression in non-functioning pituitary adenoma using formalin-fixed and paraffin-embedded tissue specimens
Relations
Alternative to GSM2053566 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
JUC01000001.hg.1 3.215368
JUC01000002.hg.1 0.8627441
JUC01000003.hg.1 5.608392
JUC01000004.hg.1 1.766291
JUC01000005.hg.1 3.368273
JUC01000006.hg.1 2.144398
JUC01000007.hg.1 1.62265
JUC01000008.hg.1 3.066651
JUC01000009.hg.1 0.9843209
JUC01000010.hg.1 1.032433
JUC01000011.hg.1 7.85357
JUC01000012.hg.1 4.997226
JUC01000013.hg.1 6.121254
JUC01000014.hg.1 2.360151
JUC01000015.hg.1 1.03706
JUC01000016.hg.1 5.329459
JUC01000017.hg.1 1.620588
JUC01000018.hg.1 4.603417
JUC01000019.hg.1 3.590837
JUC01000020.hg.1 3.590837

Total number of rows: 914585

Table truncated, full table size 23587 Kbytes.




Supplementary file Size Download File type/resource
GSM2055157_NFPA_T3.CEL.gz 19.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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