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Sample GSM2055156 Query DataSets for GSM2055156
Status Public on Feb 06, 2016
Title FFPE specimen of NFPA, biological rep2 [exon-level]
Sample type RNA
Source name obtained from archived tissue samples derived from patients with NFPA
Organism Homo sapiens
Characteristics gender: male
tissue: pituitary
age: 36 years old
Extracted molecule total RNA
Extraction protocol RNA was extracted by Trizol (Life Technology, 15596-018) following the manufacturer's instructions
Label biotin
Label protocol Total RNA were amplified, labeled and purified by using WT Terminal Labeling and Controls Kit (Cat#901524, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Hybridization protocol Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 640 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions
Scan protocol Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 4.0 (Affymetrix, Santa Clara, CA, US) with default settings.
Description Gene expression data from FFPE specimen
Data processing Affymetrix GeneChip Command Console (version 4.0, Affymetrix) software was used to extract raw data. Next, Expression Console (version1.3.1, Affymetrix) software offered RMA normalization for both gene and exon level analysis. Then the gene expression analysis and alternative splice analysis proceeded by Genesrping software (version 13.0; Agilent Technologies) and Transcriptome Analysis Console (version2.0, Affymetrix) separately.
Submission date Feb 05, 2016
Last update date Feb 06, 2016
Contact name weikang xing
Organization name The First Affiliated Hospital of Soochow University
Street address 188 shizi street
City suzhou
ZIP/Postal code 215000
Country China
Platform ID GPL17585
Series (1)
GSE77517 Genome-wide evaluation of change in lncRNA expression in non-functioning pituitary adenoma using formalin-fixed and paraffin-embedded tissue specimens
Alternative to GSM2053565 (gene-level analysis)

Data table header descriptions
VALUE RMA signal intensity

Data table
JUC01000001.hg.1 3.475774
JUC01000002.hg.1 2.549906
JUC01000003.hg.1 7.1488
JUC01000004.hg.1 1.148685
JUC01000005.hg.1 4.590462
JUC01000006.hg.1 2.745084
JUC01000007.hg.1 2.17576
JUC01000008.hg.1 2.123864
JUC01000009.hg.1 0.6935036
JUC01000010.hg.1 0.7715363
JUC01000011.hg.1 7.833603
JUC01000012.hg.1 2.944349
JUC01000013.hg.1 3.240874
JUC01000014.hg.1 3.26825
JUC01000015.hg.1 0.9667911
JUC01000016.hg.1 5.043102
JUC01000017.hg.1 1.502017
JUC01000018.hg.1 4.966788
JUC01000019.hg.1 4.241461
JUC01000020.hg.1 4.241461

Total number of rows: 914585

Table truncated, full table size 23571 Kbytes.

Supplementary file Size Download File type/resource
GSM2055156_NFPA_T2.CEL.gz 18.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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