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Sample GSM2051860 Query DataSets for GSM2051860
Status Public on Feb 02, 2016
Title HSC rep4
Sample type RNA
Source name Bone Marrow, HSC, replicate 4
Organism Homo sapiens
Characteristics tissue: Bone Marrow
gender: Male
Extracted molecule total RNA
Extraction protocol Target populations ranging in number from 5000 cells were double-sorted directly to Trizol reagent (Life Technologies). Total RNA extracted in accordance with the manufacture's instruction.
Label Cy3
Label protocol Cyanine3-labeled cRNA from each sample was prepared to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies) according to the manufacture's instruction.
Hybridization protocol cRNA (800 ng) was hybridized to the microarray chip (SurePrint G3 Human Gene Expression 8x60K v2, Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the SureScan Microarray Scanner (G4900DA) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green and Green PMT sensitivity is set to 100%).
Description Gene expression of HSC from bone marrow of healthy donor
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Raw expression data was imported and normalized by using GeneSpring GX software (Agilent Technologies)
Submission date Feb 01, 2016
Last update date Feb 02, 2016
Contact name Kohta Miyawaki
Organization name Kyushu University
Street address 3-1-1, Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platform ID GPL17077
Series (1)
GSE77439 Development of gene signatures of human unipotent megakaryocyte progenitor and other hematopoietic stem and progenitor populations

Data table header descriptions
VALUE Normalized expression

Data table
A_23_P117082 5.175274
A_33_P3246448 -5.953818
A_33_P3318220 -5.9455867
A_33_P3236322 -5.9382343
A_33_P3319925 -3.5126967
A_21_P0000509 4.563958
A_21_P0000744 3.039956
A_24_P215804 -0.974329
A_23_P110167 3.3760738
A_33_P3211513 1.3456583
A_23_P103349 -3.9251306
A_32_P61480 -5.0604863
A_33_P3788124 -5.8486996
A_33_P3414202 0.5039301
A_33_P3316686 0.9625912
A_33_P3300975 -2.2177505
A_33_P3263061 3.384509
A_33_P3261373 -5.896575
A_24_P278460 -0.016153336
A_21_P0013109 -5.898239

Total number of rows: 50737

Table truncated, full table size 1193 Kbytes.

Supplementary file Size Download File type/resource
GSM2051860_SG13284322_253949430551_S001_GE1_1105_Oct12_1_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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