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Sample GSM2051854 Query DataSets for GSM2051854
Status Public on Feb 02, 2016
Title HSC rep2
Sample type RNA
 
Source name Bone Marrow, HSC, replicate 2
Organism Homo sapiens
Characteristics tissue: Bone Marrow
gender: Male
Extracted molecule total RNA
Extraction protocol Target populations ranging in number from 5000 cells were double-sorted directly to Trizol reagent (Life Technologies). Total RNA extracted in accordance with the manufacture's instruction.
Label Cy3
Label protocol Cyanine3-labeled cRNA from each sample was prepared to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies) according to the manufacture's instruction.
 
Hybridization protocol cRNA (800 ng) was hybridized to the microarray chip (SurePrint G3 Human Gene Expression 8x60K v2, Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the SureScan Microarray Scanner (G4900DA) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green and Green PMT sensitivity is set to 100%).
Description Gene expression of HSC from bone marrow of healthy donor
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Raw expression data was imported and normalized by using GeneSpring GX software (Agilent Technologies)
 
Submission date Feb 01, 2016
Last update date Feb 02, 2016
Contact name Kohta Miyawaki
E-mail(s) kohtam@gmail.com
Organization name Kyushu University
Street address 3-1-1, Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platform ID GPL17077
Series (1)
GSE77439 Development of gene signatures of human unipotent megakaryocyte progenitor and other hematopoietic stem and progenitor populations

Data table header descriptions
ID_REF
VALUE Normalized expression

Data table
ID_REF VALUE
A_23_P117082 4.644003
A_33_P3246448 -4.538833
A_33_P3318220 -6.321005
A_33_P3236322 -5.1610985
A_33_P3319925 -3.1205544
A_21_P0000509 2.4702816
A_21_P0000744 2.763361
A_24_P215804 -1.2096748
A_23_P110167 3.2793226
A_33_P3211513 2.5479174
A_23_P103349 -2.8789368
A_32_P61480 -7.9325643
A_33_P3788124 -6.52396
A_33_P3414202 1.4703093
A_33_P3316686 1.5690289
A_33_P3300975 -1.7575626
A_33_P3263061 3.5526114
A_33_P3261373 -4.927104
A_24_P278460 0.54488564
A_21_P0013109 -7.1537085

Total number of rows: 50737

Table truncated, full table size 1191 Kbytes.




Supplementary file Size Download File type/resource
GSM2051854_SG13284322_253949427407_S001_GE1_1105_Oct12_1_1.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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