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Sample GSM2051849 Query DataSets for GSM2051849
Status Public on Feb 02, 2016
Title HSC rep1
Sample type RNA
 
Source name Bone Marrow, HSC, replicate 1
Organism Homo sapiens
Characteristics tissue: Bone Marrow
gender: Male
Extracted molecule total RNA
Extraction protocol Target populations ranging in number from 5000 cells were double-sorted directly to Trizol reagent (Life Technologies). Total RNA extracted in accordance with the manufacture's instruction.
Label Cy3
Label protocol Cyanine3-labeled cRNA from each sample was prepared to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies) according to the manufacture's instruction.
 
Hybridization protocol cRNA (800 ng) was hybridized to the microarray chip (SurePrint G3 Human Gene Expression 8x60K v2, Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the SureScan Microarray Scanner (G4900DA) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green and Green PMT sensitivity is set to 100%).
Description Gene expression of HSC from bone marrow of healthy donor
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Raw expression data was imported and normalized by using GeneSpring GX software (Agilent Technologies)
 
Submission date Feb 01, 2016
Last update date Feb 02, 2016
Contact name Kohta Miyawaki
E-mail(s) kohtam@gmail.com
Organization name Kyushu University
Street address 3-1-1, Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platform ID GPL17077
Series (1)
GSE77439 Development of gene signatures of human unipotent megakaryocyte progenitor and other hematopoietic stem and progenitor populations

Data table header descriptions
ID_REF
VALUE Normalized expression

Data table
ID_REF VALUE
A_23_P117082 4.86448
A_33_P3246448 -5.2681503
A_33_P3318220 -7.852669
A_33_P3236322 -4.78689
A_33_P3319925 -3.3287387
A_21_P0000509 3.7193403
A_21_P0000744 2.947565
A_24_P215804 -0.41240406
A_23_P110167 3.335187
A_33_P3211513 1.4483414
A_23_P103349 -4.6035266
A_32_P61480 -7.892765
A_33_P3788124 -6.0826225
A_33_P3414202 0.5989399
A_33_P3316686 0.7964983
A_33_P3300975 -2.519147
A_33_P3263061 3.2486658
A_33_P3261373 -6.2366533
A_24_P278460 0.06431675
A_21_P0013109 -7.8323474

Total number of rows: 50737

Table truncated, full table size 1192 Kbytes.




Supplementary file Size Download File type/resource
GSM2051849_SG13284322_253949427528_S001_GE1_1105_Oct12_1_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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