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Sample GSM2051847 Query DataSets for GSM2051847
Status Public on Feb 02, 2016
Title CMP rep1
Sample type RNA
 
Source name Bone Marrow, CMP, replicate 1
Organism Homo sapiens
Characteristics tissue: Bone Marrow
gender: Male
Extracted molecule total RNA
Extraction protocol Target populations ranging in number from 5000 cells were double-sorted directly to Trizol reagent (Life Technologies). Total RNA extracted in accordance with the manufacture's instruction.
Label Cy3
Label protocol Cyanine3-labeled cRNA from each sample was prepared to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies) according to the manufacture's instruction.
 
Hybridization protocol cRNA (800 ng) was hybridized to the microarray chip (SurePrint G3 Human Gene Expression 8x60K v2, Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the SureScan Microarray Scanner (G4900DA) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green and Green PMT sensitivity is set to 100%).
Description Gene expression of Common Myeloid Progenitor from bone marrow of healthy donor
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Raw expression data was imported and normalized by using GeneSpring GX software (Agilent Technologies)
 
Submission date Feb 01, 2016
Last update date Feb 02, 2016
Contact name Kohta Miyawaki
E-mail(s) kohtam@gmail.com
Organization name Kyushu University
Street address 3-1-1, Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platform ID GPL17077
Series (1)
GSE77439 Development of gene signatures of human unipotent megakaryocyte progenitor and other hematopoietic stem and progenitor populations

Data table header descriptions
ID_REF
VALUE Normalized expression

Data table
ID_REF VALUE
A_23_P117082 5.0837526
A_33_P3246448 -5.38656
A_33_P3318220 -7.955344
A_33_P3236322 -5.165921
A_33_P3319925 -3.2222886
A_21_P0000509 4.6376324
A_21_P0000744 4.3387785
A_24_P215804 -0.08493519
A_23_P110167 4.4244795
A_33_P3211513 0.7340765
A_23_P103349 -4.679528
A_32_P61480 -6.05783
A_33_P3788124 -7.2034254
A_33_P3414202 0.7570801
A_33_P3316686 1.1130486
A_33_P3300975 -2.4458604
A_33_P3263061 3.8070803
A_33_P3261373 -5.884168
A_24_P278460 0.1825018
A_21_P0013109 -7.9424214

Total number of rows: 50737

Table truncated, full table size 1189 Kbytes.




Supplementary file Size Download File type/resource
GSM2051847_SG13284322_253949427528_S001_GE1_1105_Oct12_1_4.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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