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Sample GSM2049191 Query DataSets for GSM2049191
Status Public on May 09, 2016
Title Bach2-tdRFP low 2
Sample type SRA
 
Source name IgG1 LZ GC B cells, low Bach2-tdRFP
Organism Mus musculus
Characteristics strain/background: C57BL/6
genotype/variation: Bach2-tdRFP
tissue origination: spleen
cell type: NP-specific IgG1 light zone germinal center B cells
bach2-tdrfp expression: low
Treatment protocol tdRFP low and high IgG1 LZ GC B cells were FACS-sorted from 3 Bach2-tdRFP mice and pooled for each sample.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
DNA libraries were prepared for sequencing using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description S06300
Data processing Read alignment: TopHat2 v2.0.8 with default settings.
Expression level: Cufflinks v2.1.1 with default settings.
Genome_build: mm9 (MGSCv37)
Supplementary_files_format_and_content: *fpkm.txt: Includes FPKM values.
 
Submission date Jan 27, 2016
Last update date May 15, 2019
Contact name TOMOHIRO KUROSAKI
E-mail(s) kurosaki@ifrec.osaka-u.ac.jp
Phone +81-6-6879-4458
Organization name Osaka University Immunology Frontier Research Center
Lab Laboratory of Lymphocyte Differentiation
Street address 3-1 Yamada-oka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platform ID GPL18480
Series (1)
GSE77319 Comparison of Bach2-tdRFP high expression and low expression NP-specific IgG1 light zone GC B cells
Relations
BioSample SAMN04445536
SRA SRX1550095

Supplementary file Size Download File type/resource
GSM2049191_S06300.fpkm.txt.gz 744.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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