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Sample GSM2045991 Query DataSets for GSM2045991
Status Public on Jul 18, 2019
Title CCS 1062
Sample type RNA
 
Source name surgically resected human sarcoma sample
Organism Homo sapiens
Characteristics tissue: surgically resected human sarcoma sample
Treatment protocol Dox ON samples were treated with Dox (0.2µg/ml) containing medium for indicated hours.
Growth protocol G1297; DMEM+10%FBS, PS/MEF; DMEM+10%FBS, PS, NEAA, L-Glu, 2-ME/iPSCs; Knockout DMEM+15%FBS, PS, NEAA, L-Glu, 2-ME, LIF
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit
Label Biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
Scan protocol Affymetrix Gene ChIP Scanner 3000 TG
Data processing Data were processed using Genespring software.
 
Submission date Jan 25, 2016
Last update date Jul 18, 2019
Contact name Yasuhiro Yamada
E-mail(s) yyamada@m.u-tokyo.ac.jp
Organization name University of Tokyo
Department Department of Molecular Pathology
Street address 7-3-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
 
Platform ID GPL6244
Series (2)
GSE77203 Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas
GSE77205 Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas

Data table header descriptions
ID_REF
VALUE Quantile normalized gene level expression values from Genespring GX

Data table
ID_REF VALUE
7896736 119.030235
7896738 23.051529
7896740 23.396343
7896742 523.7257
7896744 91.78885
7896746 902.68176
7896748 56.671703
7896750 26.35849
7896752 293.43976
7896754 158.27466
7896756 128.59525
7896759 128.14188
7896761 121.140076
7896779 142.14232
7896798 167.99905
7896817 112.70257
7896822 217.8454
7896859 63.760292
7896861 24.67197
7896863 71.51796

Total number of rows: 28869

Table truncated, full table size 497 Kbytes.




Supplementary file Size Download File type/resource
GSM2045991_CCS_1062_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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