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Sample GSM2040541 Query DataSets for GSM2040541
Status Public on Jul 14, 2016
Title Wiz_ChIP-seq_2
Sample type SRA
Source name Mouse_Cerebellum
Organism Mus musculus
Characteristics strain: FVB
tissue: Cerebellum pooled
age: 56 days post partum
genotype: Wiz +/+
chip antibody: Anti-Wiz NBP180586, Novus Biologicals, USA
Extracted molecule genomic DNA
Extraction protocol Cerebellum were removed and pooled from 2 mice and flash frozen on dry ice. Cerebellum were sent to Active Motif (CA, USA) who then carried out ChIP and library construction as well as Input DNA library construction (Cat No. 25001 and 25046).
ChIP libraries were prepared for sequencing using standard Illumina protocols
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
Data processing For ChIP-seq, sequenced reads were provided by the sequencing service provider (Active Motif) in a .fastq.gz format. Reads were mapped to the mouse mm10 genome, using the Bowtie2 program using default settings.
For ChIP-seq, peak calling was done using MACS2 (Version 2.1.0) by first filtering out duplicate reads then using default parameters and the following options -s 75 --bw 200 -m 10 30 –p 0.0000001. Peaks were considered significant if they had a p value of equal or less than 1E-20
For RNA-seq, sequenced reads were provided by the sequencing service provider (Australian Genome Research Facility) in a .fastq.gz format. Reads were mapped to the mouse mm10 genome using the program Tophat (version 2.0.11) with the following parameters: -I 100000 --library-type=fr-unstranded --read-edit-dist 3 --no-coverage-search --read-mismatches 3
For RNA-seq, read counts for gene exons were extracted using the program htseq-count (version 0.6.1) with the options -s no -m intersection-strict. Normalization and differnetial gene expression was assessed using the R-package DEseq, with default parameters.
Genome_build: mm10
Supplementary_files_format_and_content: For processed ChIP-seq data, Bigwig format files showing the mapped read density across the genome. For processed RNA-seq data, tab-delimited text files include raw read counts for each of the samples
Submission date Jan 15, 2016
Last update date May 15, 2019
Contact name Luke Thomas Isbel
Organization name SAiGENCI
Lab Isbel
Street address 4 North Terrace, AHMS, Lvl 9
City Adelaide
State/province South Australia
ZIP/Postal code 5000
Country Australia
Platform ID GPL19057
Series (1)
GSE76909 Widely Interspaced Zinc Finger Motifs, Wiz, binds active promoters and CTCF-binding sites and is required for normal neural function in the mouse
BioSample SAMN04417549
SRA SRX1531992

Supplementary file Size Download File type/resource 299.2 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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