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Sample GSM203289 Query DataSets for GSM203289
Status Public on Jun 22, 2007
Title R462P00324
Sample type RNA
 
Source name prostate cancer sample
Organism Homo sapiens
Characteristics Percentage of Tumor: 8%
Percentage of Stroma: 74%
Percentage of BPH: 5%
Percentage of Atrophic Gland: 13%
Extracted molecule total RNA
Extraction protocol QiaGen Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix Scanner.
Description prostate
Data processing The data were analyzed and normalized with RMA.
 
Submission date Jun 21, 2007
Last update date Aug 14, 2011
Contact name Yipeng Wang
E-mail(s) ywang@sdibr.org
Phone 8585813960
Organization name Vaccine Research Institute of San Diego
Street address
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL96
Series (1)
GSE8218 Gene expression data from prostate cancer samples

Data table header descriptions
ID_REF
VALUE RMA calculated log2 Signal intensity

Data table
ID_REF VALUE
1007_s_at 10.27417581
1053_at 6.277257859
117_at 6.812218674
121_at 9.505472555
1255_g_at 4.74592142
1294_at 8.208815693
1316_at 6.133180442
1320_at 5.76121337
1405_i_at 7.283324245
1431_at 4.584286673
1438_at 8.245514265
1487_at 7.827246286
1494_f_at 7.260909327
1598_g_at 11.06489087
160020_at 8.651587338
1729_at 8.276961345
1773_at 6.581026827
177_at 6.119276716
179_at 9.599847566
1861_at 6.579251381

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM203289.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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