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Sample GSM203281 Query DataSets for GSM203281
Status Public on Jun 22, 2007
Title R441P00225
Sample type RNA
 
Source name prostate cancer sample
Organism Homo sapiens
Characteristics Percentage of Tumor: 70%
Percentage of Stroma: 20%
Percentage of BPH: 0%
Percentage of Atrophic Gland: 10%
Extracted molecule total RNA
Extraction protocol QiaGen Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix Scanner.
Description prostate
Data processing The data were analyzed and normalized with RMA.
 
Submission date Jun 21, 2007
Last update date Aug 14, 2011
Contact name Yipeng Wang
E-mail(s) ywang@sdibr.org
Phone 8585813960
Organization name Vaccine Research Institute of San Diego
Street address
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL96
Series (1)
GSE8218 Gene expression data from prostate cancer samples

Data table header descriptions
ID_REF
VALUE RMA calculated log2 Signal intensity

Data table
ID_REF VALUE
1007_s_at 10.47268064
1053_at 6.17401719
117_at 6.687343972
121_at 9.381750905
1255_g_at 4.85754923
1294_at 8.130554217
1316_at 6.107882819
1320_at 5.629447435
1405_i_at 6.064898217
1431_at 4.549024851
1438_at 8.152294868
1487_at 7.875051373
1494_f_at 7.262512554
1598_g_at 10.47837425
160020_at 8.886354418
1729_at 8.225716942
1773_at 6.448545674
177_at 6.147711098
179_at 9.705779027
1861_at 7.179194343

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM203281.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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