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Sample GSM2028144 Query DataSets for GSM2028144
Status Public on Jan 07, 2016
Title HT29tsp53-p53 on then off- 1
Sample type RNA
 
Source name HT29-tsp53 38-32(16h)-32˚C(6h)
Organism Homo sapiens
Characteristics cell line: HT29-tsp53
cell type: Human Colorectal Adenocarcinoma Cell Line
genotype/variation: expressing a temperature sensitive variant of p53 (murine V135A p53 transgene)
temperature (38 or 32˚c): 38-32-38˚C
Treatment protocol One dish of subconfluent cells (60-70% confluent) were moved from the 37˚C incubator to a similarly regulated 32˚C incubator for 16 hours.
Growth protocol Cells were grown as adherent cultures in 5% CO2 at 37˚C in a humidified incubator.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's recommendations.
Label biotin
Label protocol 100ng of total RNA was labeled with Whole Transcript (WT) Sense Target Labeling kit as specified by the manufacturer (Affymetrix)
 
Hybridization protocol starndard Affymetrix protocol
Scan protocol Gene Chips were scanned with Affymetrix Scanner 3000.
Description expt 1 38 to 32 to 38
Data processing FlexArray 1.4.1, APT
probe group file: HuGene-1_0-st-v1.r3.pgf
meta-probeset file: HuGene-1_0-st-v1.r3.mps
 
Submission date Jan 06, 2016
Last update date Jan 07, 2016
Contact name Bruce McKay
E-mail(s) Bruce_McKay@carleton.ca
Phone 6135202600
Organization name Carleton University
Department Biology
Street address 1125 Colonel By Dr.
City Ottawa
State/province Ontario
ZIP/Postal code K1S 5B6
Country Canada
 
Platform ID GPL10739
Series (1)
GSE76575 The role of mRNA decay in p53-induced gene expression

Data table header descriptions
ID_REF
VALUE APT signal estimates from FlexArray 1.4.1

Data table
ID_REF VALUE
7892501 4.72609
7892502 4.32129
7892503 2.60566
7892504 6.59839
7892505 1.60056
7892506 3.42764
7892507 3.60811
7892508 4.64173
7892509 10.81989
7892510 2.08006
7892511 1.60629
7892512 5.39009
7892513 2.44747
7892514 9.61265
7892515 6.44825
7892516 6.62882
7892517 2.81309
7892518 2.16109
7892519 4.14055
7892520 7.62878

Total number of rows: 257430

Table truncated, full table size 3998 Kbytes.




Supplementary file Size Download File type/resource
GSM2028144_expt_1_38_to_32_to_38.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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